Journal: Frontiers in Microbiology

Article Title: Assessment of Gram- and Viability-Staining Methods for Quantifying Bacterial Community Dynamics Using Flow Cytometry

doi: 10.3389/fmicb.2020.01469

Figure Lengend Snippet: Aerobic/facultative anaerobic bacteria strains, culture media and Gram status.

Article Snippet: Escherichia coli , Proteobacteria , Enterobacteriaceae , ATCC ® 35218 TM , Canine , LB , -.

Techniques: Infection, Derivative Assay

Journal: Frontiers in Microbiology

Article Title: Assessment of Gram- and Viability-Staining Methods for Quantifying Bacterial Community Dynamics Using Flow Cytometry

doi: 10.3389/fmicb.2020.01469

Figure Lengend Snippet: Optimization of the discrimination between Gram-positive and Gram-negative bacteria with KCl supplementation. Overnight culture of the tested bacteria were adjusted to an OD 600 of 0.5. (A) Unstained bacterial species are represented. Staining was performed with the combination of Van-Bodipy/Van/WGA-647 in either presence of (B) PBS or (C) 1 M KCl in the dark at room temperature for 15 min. Gram-positive bacteria B. subtilis ATCC 23857, E. faecalis ATCC 29212, L. lactis ATCC 11454 are presented in red, orange and pink, respectively and Gram-negative bacteria K. pneumoniae ATCC 700603, S. enterica typhimurium ATCC 13311 and E. coli ATCC 35218 are presented in green, light blue and blue, respectively. Each bacterial species was stained separately and combined in one FCM plot.

Article Snippet: Escherichia coli , Proteobacteria , Enterobacteriaceae , ATCC ® 35218 TM , Canine , LB , -.

Techniques: Staining

Journal: Frontiers in Microbiology

Article Title: Assessment of Gram- and Viability-Staining Methods for Quantifying Bacterial Community Dynamics Using Flow Cytometry

doi: 10.3389/fmicb.2020.01469

Figure Lengend Snippet: Effects of PFA and ethanol fixation methods on Gram staining. Overnight culture of the tested bacteria were adjusted to an OD 600 of 0.5. (A) Unstained bacterial species are represented. Bacteria were stained in the dark at room temperature for 15 min with the combination of Van-Bodipy/Van/WGA-647 in the presence of 1 M KCl before fixation (B) or after 1 h fixation with 1% PFA (C) or 95% ethanol (D) . Gram-positive bacteria B. subtilis ATCC 23857, E. faecalis ATCC 29212, L. lactis ATCC 11454 are presented in red, orange, and pink, respectively and Gram-negative bacteria K. pneumoniae ATCC 700603, S. enterica typhimurium ATCC 13311 and E. coli ATCC 35218 are presented in green, light blue and blue, respectively. Each bacterial species was stained separately and combined in one FCM plot.

Article Snippet: Escherichia coli , Proteobacteria , Enterobacteriaceae , ATCC ® 35218 TM , Canine , LB , -.

Techniques: Staining

Journal: Infectious Diseases in Obstetrics and Gynecology

Article Title: Phenotypic and Genotypic Characterization of Extended-Spectrum Beta-Lactamases Produced by Escherichia coli Colonizing Pregnant Women

doi: 10.1155/2020/4190306

Figure Lengend Snippet: Distribution of resistance genes bla TEM , bla SHV , and bla CTX-M in 43 E. coli isolates.

Article Snippet: ATCC® 35218TM and ATCC® 25922TM E. coli control strains were used as positive controls for both beta lactamase and non-beta lactamase producing isolates, consecutively.

Techniques: Transmission Electron Microscopy

Journal: Infectious Diseases in Obstetrics and Gynecology

Article Title: Phenotypic and Genotypic Characterization of Extended-Spectrum Beta-Lactamases Produced by Escherichia coli Colonizing Pregnant Women

doi: 10.1155/2020/4190306

Figure Lengend Snippet: Dendrogram by pulsed-field gel electrophoresis patterns for 34 extended Beta lactamase (ESBL) producing Escherichia coli .

Article Snippet: ATCC® 35218TM and ATCC® 25922TM E. coli control strains were used as positive controls for both beta lactamase and non-beta lactamase producing isolates, consecutively.

Techniques: Pulsed-Field Gel, Electrophoresis